訂購信息

Synonyms
Hematopoietic growth factor KL, MGF, SCF
Accession
P21583
GeneID
4254
Source
Escherichia coli.
Molecular Weight
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 164 amino acids.
AA Sequence
EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 1 EU/μg of rHuSCF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage
This material is offered by Xybio for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
SDS-PAGE

Reference
1. Ronnstrand L. 2004. Cell Mol Life Sci. 61:2535-48.
2. Anderson DM, Williams DE, Tushinski R, et al. 1991. Cell Growth Differ. 2:373-8.
3. Brannan CI, Lyman SD, Williams DE, et al. 1991. Proc Natl Acad Sci U S A. 88:4671-4.
4. Okayama Y, Kawakami T. 2006. Immunol Res. 34:97-115.
Background
Stem Cell Factor (SCF) that binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival. Human SCF shares 79 % - 87 % a.a. sequence identity with canine, feline, mouse, and rat SCF. Furthermore, human SCF is weakly active on mouse cells.